Effect of Ball Mill Treatment on the Physicochemical Properties and ...
The effects of ball mill treatment (0, 2, 4, 6, 8, and 10 min) ... To 2 mL of protein solution, 400 μL of 1 mg/mL BPB (in distilled water) was added and mixed well. Two mL 20 mM phosphate buffer (pH ) and 1 mg/mL BPB (in distilled water) were used as a control. Samples and control were kept for 10 min at room temperature.